Scientific Evidence for Hyaluronic Acid

Hyaluronic acid

Our formulation of Rehydrate and Restore contains a hyaluronic acid complex, with an ultra-low molecular weight of 2-10kDa. This is 60 times more soluble than standard hyaluronic acid and provides quicker penetration through the layers of the skin for faster moisturisation.

The ultra-low molecular weight of this hyaluronic acid provides quicker penetration through the layers of the skin and faster moisturisation in comparison to any sodium hyaluronate, even those of very low molecular weight. The molecular size of this hyaluronic acid is optimized to reversibly bind to the CD44 cell membrane receptors, inducing an internal reaction, activating cell proliferation and metabolism.

This particular hyaluronic acid is not a salt, but directly the acid form of the hyaluronan. A hyaluronan is a hyaluronic acid molecule with a molecular weight of less than 250kDa, this molecule is so small that this form is very solid and stable. It provides quick percutaneous permeation and improvement of moisturisation. Alongside this, it stimulates fibroblast proliferation and increases skin elasticity.

Under 2kDa hyaluronans are considered too small to bind to the CD44 receptor.

From 2 – 10kDa, the bound is considered reversible, allowing for multiple stimulations.

Above 30kDa, the bound is considered as irreversible, allowing only for a single


The above graph shows our formulation compared to regular hyaluronic acid (60 to 2000kDa), this formulation permeates the layers of the skin more than twice after just 24 hours. This fast improvement is maintained over time, as shown in the graph above; after only 1-hour permeation has doubled in comparison to medium molecular weight Hyaluronic Acid. Even very low molecular weight (VLMW) displays lower performance to this formulation in identical conditions. 

In an in-vivo clinical study designed to measure skin moisturisation, Rehydrate and Resore's Hyaluronic Acid Complex induced a constant improvement of moisturisation in comparison to medium molecular weight sodium hyaluronate: with an increased moisturisation of 10.9% after just 30 minutes. This can be seen in the graph below.


In a second in-vivo study, our formulation was applied twice daily and displayed a 10% increase in skin elasticity after 60 days. Compared to a control of 0.25% ULMW Hyaluronic Acid, this formulation:

• Stimulates elastin synthesis by 35% within 24 hours.
• Decreases elastase activity by 25% within 2 hours.
• Decreases collagenase activity by 32% within 2 hours.

This is shown on the graph below.